UF ushers in a new age of engineering with a novel technology for 3-D printing the softest objects
By Cindy Spence

A dozen or so polymer jellyfish float in an aquarium in Tommy Angelini’s engineering lab, not alive but freaks of nature nonetheless, about to rock the world.

Just months ago, these jellyfish could not have existed; there was no way to make something so soft. That changed in 2014. Angelini was exploring ways to create fragile arrays of cells when he realized the contraption he had cobbled together not only did what he wanted it to do, but much, much, much more.

As experiments piled up — let’s try this, what if we try that — the contraption met every challenge, and doors long closed to scientific inquiry sprang open.

A new age of engineering, the Age of Soft Matter, had arrived.

As Angelini worked, he updated colleague and mentor Greg Sawyer on the eerily soft objects coming out of his lab. Sawyer issued a challenge.

“As engineers, you look at a jellyfish, and you say, that’s utterly impossible, how on earth could we manufacture that thing,” says Sawyer. “You want to make something? Make a jellyfish.”

Angelini did, and Sawyer says, “Now, let’s cross that off the impossible list, and get started.”

As a symbol of a new age and an engineering revolution that will change medicine and manufacturing, the no-longer-impossible jellyfish is perfect. If you can make a jellyfish, think what else you can make: cells, tissues, organs, tumors.


Before the jellyfish, there was a grant. Angelini had won a National Science Foundation Early Career Development Award, NSF’s most prestigious award for promising junior faculty, with a proposal to use the $500,000 to explore cell mechanics. To do that, he needed arrays of cells not confined to a petri dish. He needed cells in three dimensions.

“I wanted to study the basic physics of collective cell behavior,” Angelini says. “What do cells do spontaneously when driven by their own internal machinery?”

Tommy Angelini uses his lab’s refrigerator for quick calculations.

Angelini hired doctoral student Tapomoy Bhattacharjee and drew on assistance that ranged from a high school student working on a science fair project to Sawyer, a world-renowned tribologist and the Ebaugh professor in the Department of Mechanical and Aerospace Engineering. Angelini bought a see-through laminar cabinet commonly used by geneticists because it had a way to filter air. He procured a fine-tipped nozzle like those used in 3-D printers. All he needed was a way to hold the printed cells in place; in other words, something that did not exist.

In traditional 3-D printing, the nozzle deposits liquid on a platform, and it hardens layer by layer until an object takes shape. That method would not work with something as squishy as a cell, which could not harden and would not be supported in air.

Angelini needed a specialized material, one soft enough to allow the most fragile object to take shape, but strong enough to hold it in place, so it could be studied and manipulated. The substance had to be neutral, neither contaminating nor augmenting the object it cradled, and it had to be soluble so it would rinse off, leaving no trace. It was a tall order, but Angelini figured a granular gel medium might work.

You know it as hand sanitizer.

The granular gel medium is the same as hand sanitizer without fragrance or alcohol. It straddles the solid-fluid states that Angelini needed to both hold a fragile object and later release it because the substance is 99.8 percent water. The other 0.2 percent is a polymer. As the nozzle passes through, the gel heals in its wake, leaving no sign of disturbance other than the soft, printed object, visible through the clear gel.

The intended goal — studying cells in 3-D — had an unintended, but groundbreaking, consequence — the invention of 3-D printing of soft matter.

As word spread across campus, the kneejerk reaction was, “No, that isn’t possible.” As chemists, biologists, neurologists, doctors and engineers trooped through Angelini’s laboratory, Bhattacharjee demonstrated, printing jellyfish after jellyfish, filling an aquarium with polymer creatures.

“We showed them, ‘This is the science, this is how it works,’” Bhattacharjee says. “It’s fun for a physicist, it’s fun for a biologist.”

Disbelief, Sawyer says, quickly turned to, “Wow, this is really cool!” Seconds later, wheels turning, that became, “What can I do with it?”

Sawyer and Angelini had suggestions.


In 2015, the American Cancer Society estimates 1,658,370 cases of cancer will be diagnosed in the United States; 589,430 cancer patients will die. By 2020, treating cancer will cost the U.S. over $200 billion a year.

Perhaps more staggering than the statistics is cancer’s reach. Every family has a cancer story, and that makes cancer a grand challenge for medicine.

Greg Sawyer examines a small-scale, soft matter model of a brain.

For Sawyer, it’s a personal challenge as well.

In 2013, Sawyer was diagnosed with stage IV metastatic melanoma. At first he felt helpless but, being an engineer, he did his homework. He joined a new immune therapy clinical trial, had a trio of painful operations and months of intense radiation to fight the disease. When Angelini printed the jellyfish a year later, the intersection with cancer was clear.

When the College of Medicine’s Celebration of Research rolled around, they browsed the posters at the O’Connell Center to see if they could find a collaborator. They ran into cancer researcher Steve Ghivizzani.

“I listened to them talk about this new technology, and I thought, ‘That’s just crazy.’ Greg says, ‘Just think about it: If you had the ability to position cellular things in space without restrictions, what could you use it for?’”

Ghivizzani did think about it and emailed Sawyer that night. He was in.

Cancer has been a thorny research subject because it is a chameleon. A cancer cell in one person doesn’t behave like a cancer cell in another, even if the cancers are the same. Even more confounding, each tumor is heterogeneous. On its exterior, it might be gorging on nutrients and replicating rapidly. Deep inside, it may be almost starving, but still alive, waiting for nutrients before growing. With thousands of cancer cells in each tumor, figuring out how the cells interact will be a key to future cancer treatment.

Ghivizzani says a tool that could allow cancer research to take not one step, but possibly many giant steps, got him excited.

“Innovations in science can sometimes be subtle,” Ghivizzani says. “This rocks.”

Cancer researchers have been stuck for years with two models, the petri dish and the mouse.

Cancer cells are easy to grow in a petri dish. But the biology of a cell growing in a dish is different from the biology of a cell growing in a body. In the body, cancer cells compete with other cells for nutrients, exhibiting aggression, or malignancy. In a petri dish, without competition, they adapt to their environment and often lose the malignancy a researcher wants to study. Ghivizzani says about two-thirds of the genes expressed in a tumor in the body are expressed in opposite ways in a petri dish.

The other model is the mouse, but that, too, has limits. Many cancers don’t grow in mice, prostate cancer, for instance. Most mice used for cancer research also have been genetically engineered to suppress their immune system, so immune therapies cannot be studied. Cancer grows slowly in mice, if at all, and sometimes the patients succumb before the mice can be tested. To see inside the mouse, you have to sacrifice the animal and further study of the tumor.

What is needed is a three-dimensional model between a petri dish and a mouse: 3-D soft matter printing of cancer cells.

The goal is to harvest a patient’s cancer cells, print them into the granular gel, and allow them to grow. In theory, unlimited samples could be grown and an unlimited number of therapies tried. Researchers couldn’t use 10,000 mice at once, but they could grow 10,000 samples and try different approaches. From immune therapies to various drugs, all the tests could take place at once, saving critical time for the patient.

“We can use this system to learn a lot more a lot faster than we have been able to do,” Ghivizzani says. “Can you take each patient, print replicas of their tumor and then try different therapies? That’s where we want to go.”

Ghivizzani says the ability to collaborate with engineers on cancer research is key to reaching that goal. Working with Sawyer and Angelini one afternoon during a test, it looked like they would not be able to print fast enough to continue working the next day.

“Greg sat down and did the math, and they built another machine. By the next morning, they had it reconfigured to work the way we needed it to function,” Ghivizzani says. “Their ability to solve those kinds of technical problems that are not biologic . . . without that, we won’t get where we want to go.”

Sawyer, who feels the urgency of his fellow cancer patients every 100 days when he visits his doctors, says engineers are perfect partners for cancer research. By January, he says, UF engineers will equip a lab at the College of Medicine with engineering tools for new research.

“At first, cancer seemed far from engineering. But look at it; it’s a dynamic system, far from equilibrium, and maybe that’s not so strange to engineers. This is tumor engineering. Give us six months, and we can really help from an engineering standpoint.

“Ten years from now, I think this will be the way researchers work with patients’ cancers,” Sawyer says.


A new age of engineering creates a lot of work, and Sawyer and Angelini were eager to share the cool, new science. Department Chair David Hahn agreed to a Soft Matter Engineering Research Group, and the scientists who witnessed the first jellyfish demonstrations signed on. Today, there are 28 collaborators, including two Sawyer protégés at the University of Illinois Urbana-Champaign and Florida International University.

“Tommy made that breakthrough, printing in the granular gel, that was the quantum step forward,” Hahn says. “Now there’s a thousand things to do on top of that with the subtleties of the materials, applications, the science behind it, understanding how these materials function. It’s huge.”

Teams of engineers and doctors will tackle four areas: mechanics and failure theories of soft matter; soft matter manufacturing; transport of materials through soft matter; and soft matter for medicine.

The objects printed in the lab are orders of magnitude softer than any man-made object. Angelini says he doesn’t know of a lower limit to the mechanical integrity of the objects the lab can make, and Hahn says new mechanics and failure theories will be needed; what works for titanium will not work for cells and polymers a million times softer.

“In simple terms, a hundred-plus years that we’ve built a foundation on in traditional mechanics is largely off the table with soft matter,” Hahn says. “It really is a whole new frontier of engineering.”

Floating jellyfish

Angelini’s lab did informal tests. One early jellyfish was placed in a jar and examined weekly. It held up just fine until, at the seven-month mark, it was knocked off a shelf. On impulse, Angelini tossed a container of polymer jellyfish in his backpack to travel to a conference in Germany. They survived, so he sent them by FedEx to a colleague in Cleveland and had them shipped back. The jellyfish were no worse for wear.

Now it’s time for science to back up — or disprove — the anecdotes.

Sawyer, the go-to guy for expertise on friction and wear in mechanics and materials, says what engineering has lacked from the Stone Age to the Plastics Age and beyond is softness; soft materials have always confounded engineers.

“What is soft matter? Actually, it’s everything; it’s most of the human body. The hard matter is the weird stuff. We’ve worked with it since the Stone Age because we knew the rules,” Sawyer says. “Now, we’ve entered the Soft Matter Age.”

Engineering Professor Kevin Jones, who views materials science through a broad lens that includes anthropology, the classics, humanities and history, is creating a soft matter module for an interdisciplinary course he coordinates, “The Impact of Materials on Society.” When he wrote the course flyer, one of the teasers was, “What future materials innovations will revolutionize your world?”

“I didn’t realize that at that very moment, one of those revolutions was taking place across campus,” Jones says.

That there is no textbook for this field that didn’t exist six months ago is an opportunity, not a problem, Jones and Hahn agree.

“This has a huge wow factor. Students who thought of engineering as cars and gears, can see that mechanical engineering can be about tissue scaffolds and artificial organs and other cool stuff,” Hahn says. “The jellyfish, it’s a vehicle for excitement.”


No one is waiting for theories. Across campus, scientists are making soft materials at such a pace that six patents are pending on a technology not yet a year old.

“We had no way to make materials so soft in the past and no reason to study it. Why study something so soft and gooey you couldn’t use it for anything?” Jones says. “The minute you can engineer something out of it, that changes everything.”

The lead in manufacturing is Curtis Taylor, assistant professor of mechanical and aerospace engineering. Already, Taylor is close to solving one of the first questions raised by the new technology: Is it possible to print efficiently with more than one material? Early experiments involved printing with a single material, in most cases polymers or cells. What if you wanted to print an object with different textures at different layers — a phantom brain, for instance, with a tumor under the surface?

Taylor and a team of students tackled that problem. They came up with an acrylic tube with four ports, one for the granular gel medium and three for other materials. To avoid the need for a valve to shut off one port so another can open, they came up with an ingenious solution: use the granular gel, which easily transitions from fluid to solid, as a plug.

The device is the size of a piece of bubblegum and would cost just $2. To make cellular or sterile materials implanted in humans the device is disposable, and although the prototype is acrylic, biodegradable materials work, too. As a manufacturing innovation, it looks simple, but it’s a workhorse.

“This little device replaces four nozzles, four pumps, four different needles, four different mechanisms for putting the needles into the gel,” Taylor says.

Also in the works are larger machines for larger objects and automation with robotic arms that can build from any angle. Questions remain, including the sequence of printing — inside out or outside in — and how fast it is possible to print with precision. To answer them, Taylor is actively recruiting. “I need a student,” he says, “I need a band of students.”

The printed soft materials can mimic the softness of human body parts, like the brain, and that opens new avenues for medical education, says neurosurgery professor Frank Bova.

A prototype of a hollow, flexible tube that represents on-demand, patient-specific medical devices.

By manufacturing artificial brains and other phantom organs that students can practice on, medical education could take a leap forward, Bova says. Models of bones and cartilage have been readily available for practice, but not soft organs.

One problem with teaching medical students is having the right case at the right time. Often, complicated cases come in when a student is learning more basic methods. Bova envisions a library of phantoms, easily printed as needed, giving students cases appropriate for their skill level as their skills grow.

Other phantoms could benefit personalized medicine. A patient’s brain could be scanned and printed with its own personal architecture — tumors, blood vessels, neurons — allowing the surgeon to rehearse before operating on the patient.

Angelini says, “Can you imagine, hearing your surgeon say, ‘Good morning, I’ve practiced this surgery on your brain five times already. You’re in good hands.”

Some colleagues are stunned by the simplicity of his idea and how easily it adapts to different uses. But that’s engineering, Angelini says, the best solutions are simple and elegant.

“Every time we thought of a new thing to do with this technology, it turned out to be really amazing. Every time we thought of a new task or a new test, the material we were using turned out to have a fantastic solution,” Angelini says.

The jellyfish — the impossible jellyfish — was almost too easy, but the road ahead holds challenges.

“I think we will help people,” Sawyer says. “This may not help me, but it will help somebody.

“We’re engineers, and engineers, regardless of what it is, feel there’s got to be something we can do to effect change. There’s a certain amount of pleasure you get from just being in the fight. I like that.”

Related Website:

This article was originally featured in the Fall 2015 issue of Explore Magazine.

樱花视频 小蜜桔app破解版 麻豆影视 小草在线视频免费观看视频 大杳蕉伊在线看中文字 丝瓜丝瓜视频看片在线观看 PR18 意淫强奸 下载蘑菇视频并安装 9uu在线 烈火动漫在线完整观看 菠萝蜜视频 樱花直播最新名字 欧美爆乳乱妇高清免费 磁力天堂在线 中文字幕乱码高清在线播放 污污的视频带疼痛声的视频免费 羞羞漫画在线漫画网免费 蜜芽网站miya在线视频免费 嘿嘿连载app 富二代视频app无限观看污版在线 爱你爱你直播 蜜桔视频下载官方app 浮力路线1路线二线路三 麻豆传媒直播官网视频 红杏网 bilibili网页版入口 天天高清免费特色大片 国语自产精品视频在线看 和搜子居住的日子2国语中字 qksp秋葵视频男人加油站草莓视频 天仙tv在线 2020精品国产福利观看 好吊妞无缓冲视频观看 日本AV免费一区二区三区播放 波多野结 42部无码 麻豆影视传媒网站在线 龙猫网 靠逼视频 9uu下载 彩云直播app下载 芭乐apP下载 朵朵直播 2020国产AV在线观看大全网站 浮力路线1路线二线路三 小草免费视频 真实摄像头监控夫妇视频 国产a在线不卡片 男女性高爱潮免费视频 年轻人手机在线观看视频 gegegan 很黄很黄的激吻视频 美妙天堂免费视频 暖暖视频免费播放完整版日本 污软件 国产chinese男同志movies 半夜小旅馆嫖妓少妇 生肉动漫免费观看 9悠悠 2345影视大全最新免费观看 天仙TV 禁止的爱善良的小峓子 а天堂高清在线观看 神马达达兔 s8官网在线观看 页面升级系统自动更新 男生和女生肌肌对肌肌完整视频 言教授要撞坏了在线全文免费 国产高清在线a视频大全 东京热大战黑人合集 菠萝蜜视频在线播放菠萝 特级婬片黑美女高清视频 爱你爱你直播 曹留社区2019地址一二三四五 欧美伦禁片在线播放 神马达达兔 33ee8ee33ee色欲 烈火动漫在线完整观看 青青国产线免观看手机版 色列工口里番动画全彩 很黄很黄的激吻视频 国产色青青视频在线观看 意淫强奸 九九影视 水果视频下载 baff91cc在线观看 新版猫咪社区官网下载 91app 青草草在线视频免费观看 三级潘金莲完整版国语 麻豆在线视看视频 可以看大秀的盒子 成都4片p完整 橙子直播app下载 韩国无遮挡十八禁视频 烈火动漫大全在线观看 素人无码国产 在线观看 小草2019最新官网 亚洲BBw性色大片 麻豆传媒映画 深夜影院免费版 丝瓜视频在线 app下载 少女导航亚洲精品导航 秋葵视频下载App 富二代app官网最新版下载 免费盒子直播大全下载 樱花直播app官方安装下载 制服丝袜电影在线看片高清 老湿机免费体检1 2019天天爱天天做在线观看 靠逼视频 富二代app下载 歪歪漫画免费看 豆奶短视频下载ios版污 五五影视高 宅男网 任你操视频 爱你爱你直播 site:www.ahlfcl.com 少妇高潮免费视频 烈火动漫在线观看 国产精品在线视频 嘿嘿连载 爱情鸟视频论坛 富二代app下载安装ios免费 安装下载软件黄色片全视频 蓝雨6080 学生真实初次破初视频网站 浮力路线1路线二线路三 一边揉一边摸下身视频 9UU有你有我足矣app安卓版 扶老二国内载点1 阿娇囗交13分钟 按摩师给了我7次高潮 国产精选学生视频 老湿网 久青草视频免费视频 美国十次 污app软件免费下载app 富二代短视频f2抖音app 武松睡潘金莲三级 2019天天爱天天做在线观看 光根电影院手机观看 欧美s s s ADC影院 女人张脚让男人桶免费 草莓app芭乐视频丝瓜 小草视频com 班上的男生 我胸和下面 烈火动漫高清视频在线观看 红色—集片 樱桃污成视频人app下载安装 朵朵直播 中国幼儿开处 2020聚合直播云盒 向日葵app污 蜜桔视频下载官方app 樱桃视频污免费版app下载 蜜芽网站miya在线视频免费 MM131叭叭后进福利免费 成都吴施蒙免费 bilibili网页版入口 樱桃成视频人APP在线观看 swag圣诞节礼物完整版 最新国产在线精品视频观看 荡乳欲妇动漫 鬼1一17集动漫在线观看 磁力链bt磁力天堂 木瓜视频看的电影大全 台湾swag完整视频在哪看 f2富二代app污短视频 福利在线观看1000集 言教授要撞坏了在线全文免费 免费直播app破解版视频 小草影视免费视频大全 年轻人手机在线观看视频 2020国产在视频线自在拍 豆奶app成版人抖音网址 页面升级系统自动更新 冈本视频app下载网站 swag麋鹿 一一在线影院 蜜桃成熟时1997在线观看视频 swag台湾官网app下载 668电影 国产精品在线视频 麻豆传媒视频在线观看 茄子视频App 草莓app下载污污的视频 成都私人影吧 龙猫网 site:www.rpbaby.cn 玩吧今天老师就是你的人 OK电影天堂 小说区图片区视频区偷拍区 水咲ローラ无码版在线视频 页面升级 免费第一二三四区 小说区图片区视频区偷拍区 图片 卡通 偷拍 欧美 视频 草莓APP污 91app 很黄很黄的激吻视频 小草视频高清在线观看免费 丝瓜视频下载安装app永久 浪货国产在线播放 国语自产拍在线观看学生app 一进一出gif试看免费午夜 光根电影院手机观看 芭乐app下载大全 女女互慰潮喷在线观看 污污的视频带疼痛声的视频免费 草草影院 国产 日本 一本大道无线高清 茄子直播 无限资源在线观看 狼人香蕉香蕉在线28视频2019 富二代app下载 丝瓜丝瓜视频看片app在线观看 天堂Av亚洲欧美日韩国产综合 亚洲欧美图片手机观看 八戒八戒午夜视频无码 77女神网络 黄色网站下载 龙猫网 丝瓜视屏下载 吃哺乳期人妻的奶水 豆奶短视频.app免费 班上的男生 我胸和下面 8090电影 chinesehomemadevideo 成都4片p视频图片 别急今晚让你弄个够 飘零电影网 成人短视频 中文乱码字幕无线观看2019 福利在线观看1000集 杏红视频 在线播放五十路乱中文 女性各种B型视频 OK电影天堂 暖暖视频日本在线观看免费 秋霞2019理论2018年成片李勇局 骚虎网站 麻豆视频app 免费盒子直播大全下载 女人本色高清在线观看 达达兔达达兔Dadatu swag在线云播放 食色豆奶app在线 蜜桃成熟时在线看免费 baff91cc在线观看 手心影视 PR18 国产精品人妻在线视频 正在播放公盯着我的内裤看 富二代app下载安装ios免费 歪歪漫画免费 扶老二国内载点1 五十路六十路老熟妇A片 五月直播live app 神马影院 达达兔wpjo 猫咪社区官网 亚洲 自拍 在线 中文 自拍 被同桌摸出水来了好爽的视频 亚洲自拍偷拍 丝瓜视屏下载 卡哇伊直播app下载地址二维码 а天堂高清在线观看 女人是男人的未来一分17秒 siguaapp f2富二代app官网下载安装 猛虎视频app试看 猛虎视频app试看 国模高清炮交150p tube6 水咲ローラ无码版在线视频 五十路六十路老熟妇A片 嘿嘿连载 强婬卖婬影院 我的电影网 麻豆传媒在线观看一区 泽艺电影 向日葵污视频下载污 媚妹秀 禁止的爱善良的小峓字中字在钱免费观看 一级午夜不卡片在线视频 老湿机免费体检1 超碰免费视频 麻豆在线观看 香蕉视频app下载 光根电影院手机观看 丝瓜视频在线视频APP下载 新快喵app下载官网 亚洲性线免费观看视频成熟 麻豆视频系列在线国产 污污软件软件大全 md.pud 麻豆传媒大全 swag圣诞节礼物完整版 芭乐apP下载 大香线蕉视频伊人99 樱花社区在线观看 小欧国产在线 上色的视频软件 菠萝蜜欧美视频免费观看 向日葵视频下载app视频污 男的把j伸进女人下面免费 9悠悠 性欧美欧美巨大免费 小蝌蚪视频 国产а∨福利视频在线 老司机在线ae85 成都4视频完整版在线观看 国产亚洲\精品福利 男女性高爱潮视频免费观看 武松睡潘金莲三级 A版红楼梦正片 男女性动态激烈动全过程 k频道导航 亚洲精品自拍学生 非会员一分钟做受视频试看 千层浪视频污app下载 向日葵视频官网 三级 丰满 人妻 少妇 茄子下载app污免费 污软件 8090电影 男生和女生肌肌对肌肌完整视频 台湾swag视频 小草 视频 观看 播放 久久在线观看 草莓成视频人app免费下载集 国产女厕偷窥系列在线视频 叶子影院 www.5.app视频 http://md.pub 国产中文三级全黄 51vv视频社区福利 天天高清免费特色大片 公下面好大很粗好爽 黄站 青青国产线免观 歪歪漫画免费看 Chinese homemade video 红猫大本营点击进在线看 向日葵污视频下载污 天天高清免费特色大片 年轻人片在线观看 中国妇女去厕所24 歪歪漫画-动漫漫画首页免费的 蜜桃成熟时3在线观看手机 污软件草莓app在线观看 久re在线精品观看9 武松睡潘金莲三级 麻豆在线观看 小草2019 阿娇囗交13分钟 榴莲app最新版安装 精品久久久久久中文字幕 歪歪漫画免费看 生肉动漫免费观看 福利APP youjizzxxx 丝瓜网站 富二代视频app无限观看污版在线 曹留社区2019地址一二三四五 食色APP 初恋视频在线观看免费观看 小草播放在线视频 午夜日本大胆裸艺术 男女性高爱潮免费视频 麻豆视频app官网 77女神网络 国产chinese男同志movies 盘她直播app下载ios最新 km_v1.0.2.apk 5.04M 国产真实学生在线观看 茄子视频官网app官网下载免费 最全的欧美大片 麻豆在线视看视频 最新国产亚洲亚洲精品视频 茄子下载app污免费 swag视频在线观看高清 丝瓜app下载 暖暖高清视频在线观看大全 男女性动态激烈动全过程 豆奶短视频下载ios版污 菠萝蜜视频在线播放菠萝 2020国语自产拍在线播放 f2富二代app官网下载安装 桃花族论坛 茄子短视频app污版下载地址 食色APP 名优馆app安卓官网 小欧国产在线 豆奶抖音短视频app污 秋葵app下载ios版官网 黑帽门 穿着裙子在野战456免费视频 成版人豆奶视频app 麻豆传媒直播app官网 美女散尿频视频 丝瓜视频黄污草莓 男女性高爱潮视频一级 神马电影我不卡4K手机高清 亚洲 欧美 另类 中文 在线 有你有我足矣最新个人网页 九九影视 富二代app安卓下载 旧版本 妈妈的朋友2免费观看完整版 视频 不穿内裤的老师 成年禁看视频免费 菠萝视频 桃花族论坛 100发饮精吞精在线播放 陈冠希门事件全套视频 暖暖视频免费播放完整版日本 李综瑞60集视频全集 按摩师给了我7次高潮 f2富二代app官网下载安装 肥胖老人做受免费视频 成版人抖音五月天 菠萝蜜欧美视频免费观看 秋霞电影免费鲁丝片观看 新五十路熟妇在线视频HD 男女交性视频播放 site:www.szgulou.com 香蕉视频污下载app污官网 国产女厕偷窥系列在线视频 99国产这里有精品视频 芒果视频app下载污免费观看ios 大屁股村妇浪水多 泡芙视频如何无限观影 草莓视频丝瓜视污 adc影院 丝瓜app下载免费下载 国产拍国产拍拍偷 chinesehomemadevideo 浪货国产在线播放 我的电影网 妈妈的朋友3在线线观中文免费观 台湾麻豆传媒app官网版 磁力天堂在线 向日葵视频下载app视频污 秋葵视频.apk下载下载ios版 盘她直播app下载ios最新 玩吧今天老师就是你的人 小草社区视频在线 水蜜蜜视频 依依电影 女人与公拘交的视频网站